![]() ![]() A decolorizer made of acetone and alcohol (95%).Then when again stained with safranin, they take the stain and appears red in color. When they are exposed to alcohol, decolorizer dissolves the lipids in the cell walls, which allows the crystal violet-iodine complex to leach out of the cells. In case of gram negative bacteria, cell wall also takes up the CV-Iodine complex but due to the thin layer of peptidoglycan and thick outer layer which is formed of lipids, CV-Iodine complex gets washed off. So the ethanol cannot remove the Crystal Violet-Iodine complex that is bound to the thick layer of peptidoglycan of gram positive bacteria and appears blue or purple in color. Decolorizing the cell causes this thick cell wall to dehydrate and shrink, which closes the pores in the cell wall and prevents the stain from exiting the cell. The cell walls of gram positive bacteria have a thick layer of protein-sugar complexes called peptidoglycan and lipid content is low. When the bacteria is stained with primary stain Crystal Violet and fixed by the mordant, some of the bacteria are able to retain the primary stain and some are decolorized by alcohol. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms. Crystal violet is purple and gives the characteristic color to the bacterial culture, which helps differentiate the two types of gram bacteria.Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. The major dye responsible for staining the bacteria is crystal violet stain. Gram-negative bacteria lack peptidoglycan therefore, they do not retain the gran stain and appear red to pink after decolorization with ethanol. The gram stain dye is retained in the cell wall due to the thickness of peptidoglycan present in gram-positive bacteria. The major difference between gram-positive and gram-negative bacteria is the difference in their cell walls. The difference between gram-positive and gram-negative bacteria Conclusion. The results are due to the bacteria's cell wall structure difference. If the bacterial culture appears pink to red, it is gram-negative bacteria. If the bacterial culture appears purple, then it is gram-positive bacteria. Gram staining procedure - Observation and results -Īfter performing the staining procedure, the scientists observe the culture's color under the microscope. Examining the culture under the microscope.Processing of the sample through a series of steps.Collection of bacterial samples from the infection site.The different steps in the gram staining technique are as follows. After performing all the technique steps, the cells are observed under the microscope. The medical laboratory scientist put the samples on the glass slide and applied a series of stains to the bacterial culture. It is then contained in a sterile container to send to the laboratory. The sample can be in the form of a swab or mucus. The sample of bacteria is taken from the site of infection. Many infections, like pneumonia, urinary tract infections, etc., are diagnosed using gram staining. It is used as a first step in giving the right direction for identifying bacteria. ![]() ![]() However, it is inefficient to diagnose the specific bacterium present in the culture, which is the main cause of the disease. This technique is quicker than most other bacterial culture techniques. Upon the identification of the color of the bacterial culture, they are differentiated as gram-positive or gram-negative. The bacterial culture is put on a glass slide and observed under a microscope. The technique was named after his name, and he developed this technique while working on pneumonia.īacterial cultures are used to diagnose the type of bacteria present. The technique was developed by a famous bacteriologist, Hans Christian Gram, in 1882. According to this technique, the two types of bacteria are stained, red or purple, based on their type of cell wall. The technique is relatively quick and helps health practitioners identify the bacteria at the site of infection. Both types of bacteria have different cell wall structures, and the ability of the bacterial cell wall to retain the dye color differentiates gram-positive from gram-negative bacteria. The technique involves staining the bacterial cell with certain dyes. The two types of bacteria are differentiated based on a microbial technique called gram staining. There are two types of bacterial infections caused by two different types of bacteria - gram-positive and gram-negative. Next → ← prev Difference Between Gram-Positive Bacteria and Gram-Negative Bacteria ![]()
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